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A ⁵¹Cr Microassay Technique for Cell-Mediated Immunity to Viruses¹

From the Departments of Pediatrics and Microbiology, Georgetown University School of Medicine, Washington, D. C.; Microbiological Associates, Bethesda, Maryland; and National Institutes of Health, NINDS, Infectious Diseases Branch, Bethesda, Maryland

Abstract

A ⁵¹Cr microassay procedure for the in vitro measurement of cell-mediated immunity to rubella virus is described. The technique consists of a lymphocyte target cell interaction employing tissue culture lines chronically infected with rubella virus in which the quantitative release of ⁵¹Cr is used as an index of lymphocyte reactivity. Also described in these studies is an apparatus which separates the reacting cells from supernatant fluids and recovers the released ⁵¹Cr. Significant release is only observed with lymphocytes from rubella seropositive individuals and the reaction is inhibited by rubella hyperimmune sera. The microassay technique also appears to be applicable for the study of cellular immunity to a wide variety of viral agents.

Footnotes

¹ This work was supported in part by United States Army Medical Research and Development Command DA-49-193-MD-2633 and NIH Training Grant HD-00261.

² Department of Pediatrics and Microbiology, Georgetown University School of Medicine, Washington, D. C.

³ Microbiological Associates, Bethesda, Maryland.

⁴ National Institutes of Health, NINDS, Infectious Branch, Bethesda, Maryland.