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Biosynthesis and Secretion of Antibody by Chicken Lymphoid Cells¹

From the Pathology Research Laboratories, Variety Club Heart Hospital and Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455

Abstract

A method to study biosynthesis and secretion of antibody by chicken lymphoid cells has been developed. Briefly, antibody was labeled with leucine-³H by incubating immunized chicken lymphoid cells in a short-term tissue culture, and was purified from other cellular proteins by specific immune adsorption to polymerized antigen. Biochemical structure of antibody molecules purified above was studied by sodium dodecyl sulfate-acrylamide gel electrophoresis.

Using this method, we demonstrate that spleen cells of the chicken actively synthesize and secrete antibody which was characterized as being an immunoglobulin by serology and acrylamide gel analysis. However, immunoglobulin antibody is not detectable in bursa cells by this method regardless of the routes of bovine -globulin immunization.

Footnotes

¹ This work was supported by United States Public Health Service Grant AI-10415, and by grants from the American Cancer Society, Damon Runyon Memorial Fund, and The National Foundation—March of Dimes.

² Supported by United States Public Health Service Career Development Award GM-44268.