HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Patterson, R. Articles by McIntire, F. C. Search for Related Content PubMed Articles by Patterson, R. Articles by McIntire, F. C.

Polymerized Ragweed Antigen E

I. Preparation and Immunologic Studies¹

From the Allergy-Immunology Section Department of Medicine, Chicago Wesley Memorial Hospital and Northwestern University Medical School and Division of Experimental Biology, Abbott Laboratories, North Chicago, Illinois

Abstract

Glutaraldehyde treatment of partially purified ragweed AgE was done to provide cross-linked proteins of this material. The ragweed preparation contained a high concentration of antigen E. The glutaraldehyde treatment produced aggregates of ragweed antigen E that were fractionated through Sepharose 6B, 4B and Sephadex G-200 to prepare polymers of two molecular weight ranges, the first approximately 0.2 to 4 million and the second 4 to 20 million. These materials reacted completely with antibody against ragweed antigen E but required higher concentrations to neutralize antibody as the molecular weight of polymerized ragweed increased. The polymerized ragweed did not show antigenic deletion nor were new antigenic determinants definitively shown. The polymerized ragweed was at least as antigenic in animal studies as ragweed antigen E and the polymerized ragweed antigen with a molecular weight range of approximately 0.2 to 4 million showed evidence of possibly greater antigenicity than the monomeric ragweed antigen E.

Footnotes

¹ This work was supported by the Ernest S. Bazley Grant.

This article has been cited by other articles:

				I. P. Lewkowich, J. D. Rempel, and K. T. HayGlass Prevention of Allergen-Specific, Th2-Biased Immune Responses In Vivo: Role of Increased IL-12 and IL-18 Responsiveness J. Immunol., October 15, 2005; 175(8): 4956 - 4962. [Abstract] [Full Text] [PDF]