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Lymphoid Cell Dependence of Eosinophil Response to Antigen

III. Comparison of the Rate of Appearance of Two Types of Memory Cells in Various Lymphoid Tissues at Different Times After Priming¹

From the Department of Anatomy, Downstate Medical Center, Brooklyn, New York 11203

Abstract

Priming induced at least two distinct types of memory cells as determined by their capacity after adoptive transfer to elicit secondary type eosinophil or antitoxin responses to a challenging injection of tetanus toxoid. Thymic depletion prevents the formation of both types of memory cells, and injection of thymic cells 1 week before priming restores the capacity of these animals to respond. Memory cells involved in eosinophil responses are formed earlier and become more widely distributed in the blood and lymphatic tissues than do memory cells involved in antitoxin responses. Beginning on or about 6 days after priming and in all periods examined thereafter, eosinophil responses were obtained with cell suspensions of all lymphatic tissues tested. Inflammatory exudates obtained from the peritoneal cavity 7, 11, and 13 days after subcutaneous priming contained cells capable of augmenting the cellular responses but not the secondary type antitoxin responses. Memory cells capable of inducing antitoxin responses were detected in the regional lymph node suspensions 10 days after priming. At 17 days the regional lymph node and, to a lesser extent, other lymph nodes and the spleen induced antitoxin responses. By 30 days all lymphatic tissues tested except the thymus gave good responses.

We discuss the possible role of eosinophils in immunologic reactions and suggest that eosinophils are an integral component of the anamnestic responses to antigen.

Footnotes

¹ This work was supported by United States Public Health Service Grant 5 T01 GM 00379-12. A preliminary report of this work was presented at the Federation of American Societies for Experimental Biology, April 1972.

² Submitted in partial fulfilment for the degree of Doctor of Philosophy in the Department of Anatomy, Downstate Medical Center.

³ Career Scientist of the Health Research Council of the City of New York.