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The Journal of Immunology, 1973, 110: 1222-1232.
Copyright © 1973 by The American Association of Immunologists, Inc.
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A Rabbit Antiserum to a Thymus Extract Specific for Mouse Thymus-Derived Cells1

Siddhartha Sarkar2, Robert Hyman3, Tohru Masuda and Leonard A. Herzenberg

From The Salk Institute, P.O. Box 1809, San Diego, California 92112, and the Department of Genetics, Stanford University School of Medicine Stanford, California 94305

Abstract

A heteroantiserum has been prepared in rabbits against an EDTA eluate of mouse thymus. The globulin fraction of the unabsorbed serum precipitates macromolecules of several molecular weights eluted from various lymphomas. It is highly cytotoxic to both Thy-1 ({vartheta})-positive lymphomas and normal thymus cells, but is also cytotoxic to some bone marrow cells. After absorption with insolubilized serum proteins and bone marrow cells, the serum is highly specific for mouse thymus-derived cells.

Indirect immunofluorescence of thymus cells assessed by rapid-flow fluorescent measurements gives a single sharp distribution; the activity of thymusderived cells is specifically removed from primed spleen cells by the absorbed serum plus complement when tested in an in vivo transfer system. The antiserum does not discriminate between Thy-1 ({vartheta}) alleles.

Footnotes

1 This work was supported by grants from the American Cancer Society, Inc., and its Californis Division to S. Sarkar, The Armand Hammer Fund for Cancer Research to Robert Hyman, United States Public Health Service Grants AI05875 and CA05212 to M. Cohn and AI08917 and GM17367 to L. A. Herzenberg.

2 Dernham Senior Fellow of the American Cancer Society, California Division.

3 Special Fellow of the Leukemia Society.






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