HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miller, J. N. Search for Related Content PubMed Articles by Miller, J. N.

Immunity in Experimental Syphilis

VI. Successful Vaccination of Rabbits with Treponema Pallidum, Nichols Strain, Attenuated by -Irradiation¹

From the Department of Medical Microbiology and Immunology, School of Medicine, University of California, Los Angeles, California 90024

Abstract

Twenty-four rabbits, each immunized intravenously over a 37-week period with a total of 3.71 x 10⁹ -irradiated, non-infectious Treponema pallidum, Nichols strain, were found to be completely resistant to intradermal challenge with either 1000 or 100,000 homologous treponemes administered 10 days after the last immunizing injection. Persistence of complete immunity was demonstrable in each of 11 vaccinated rabbits challenged intradermally with 1800 T. pallidum, Nichols strain, 1 year after the last immunizing injection. In contrast, only minimal resistance was observed in 10 of the T. pallidum-immunized rabbits challenged intradermally with 1000 Treponema pertenue, Haiti B strain, 14 days after completion of immunization.

Venereal Disease Research Laboratory, Treponema pallidum immobilization (TPI), and Fluorescent Treponemal Antibody-Absorbed antibodies, each of which is important in the diagnosis and control of syphilis, developed during immunization and persisted in some animals for at least 1 year.

Conclusive evidence that TPI antibody is not associated with the immune response was provided by the finding that 8 of the 11 immune rabbits challenged 1 year after vaccination had no TPI antibody before challenge and failed to develop immobilizing antibody during the 3-month period of observation after challenge.

Footnotes

¹ These studies were supported by United States Public Health Service Research Grant CC-00115 from the National Center for Disease Control, Atlanta, Ga., and by the World Health Organization.

This article has been cited by other articles:

				M. B. Brinkman, M. A. McGill, J. Pettersson, A. Rogers, P. Matejkova, D. Smajs, G. M. Weinstock, S. J. Norris, and T. Palzkill A Novel Treponema pallidum Antigen, TP0136, Is an Outer Membrane Protein That Binds Human Fibronectin Infect. Immun., May 1, 2008; 76(5): 1848 - 1857. [Abstract] [Full Text] [PDF]

				M. B. Brinkman, M. McKevitt, M. McLoughlin, C. Perez, J. Howell, G. M. Weinstock, S. J. Norris, and T. Palzkill Reactivity of Antibodies from Syphilis Patients to a Protein Array Representing the Treponema pallidum Proteome. J. Clin. Microbiol., March 1, 2006; 44(3): 888 - 891. [Abstract] [Full Text] [PDF]

				R. E. LaFond and S. A. Lukehart Biological Basis for Syphilis Clin. Microbiol. Rev., January 1, 2006; 19(1): 29 - 49. [Abstract] [Full Text] [PDF]

				M. McKevitt, K. Patel, D. Smajs, M. Marsh, M. McLoughlin, S. J. Norris, G. M. Weinstock, and T. Palzkill Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery Genome Res., July 1, 2003; 13(7): 1665 - 1674. [Abstract] [Full Text] [PDF]

				C. A. Morgan, S. A. Lukehart, and W. C. Van Voorhis Immunization with the N-Terminal Portion of Treponema pallidum Repeat Protein K Attenuates Syphilitic Lesion Development in the Rabbit Model Infect. Immun., December 1, 2002; 70(12): 6811 - 6816. [Abstract] [Full Text] [PDF]

				R. Lux, J. N. Miller, N.-H. Park, and W. Shi Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola Infect. Immun., October 1, 2001; 69(10): 6276 - 6283. [Abstract] [Full Text] [PDF]

				K. Wicher, V. Wicher, F. Abbruscato, and R. E. Baughn Treponema pallidum subsp. pertenue Displays Pathogenic Properties Different from Those of T. pallidum subsp. pallidum Infect. Immun., June 1, 2000; 68(6): 3219 - 3225. [Abstract] [Full Text] [PDF]

				C. E. Cameron, C. Castro, S. A. Lukehart, and W. C. Van Voorhis Sequence Conservation of Glycerophosphodiester Phosphodiesterase among Treponema pallidum Strains Infect. Immun., June 1, 1999; 67(6): 3168 - 3170. [Abstract] [Full Text] [PDF]

				C. E. Cameron, C. Castro, S. A. Lukehart, and W. C. Van Voorhis Function and Protective Capacity of Treponema pallidum subsp. pallidum Glycerophosphodiester Phosphodiesterase Infect. Immun., December 1, 1998; 66(12): 5763 - 5770. [Abstract] [Full Text] [PDF]