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Reconstitution of T Cell-Depleted Spleen Cell Populations by Factors Derived from T Cells

I. Conditions for the Production of Active T Cell Supernatants¹

From the Department of Medical Biophysics, University of Toronto and The Ontario Cancer Institute, Toronto, Ontario, Canada

Abstract

At least part of the interaction between bone marrow-derived (B) and thymus-derived (T) lymphocytes during the initiation of an in vitro immune response to sheep erythrocytes (SRBC) is mediated by a nonspecific activity released from antigen-stimulated T cells. The release of the activity requires stimulation with specific antigen, but the activity produced by different antigens can reconstitute an in vitro response to SRBC. The assay used to investigate the activity has been the reconstitution of spleen cell populations treated with anti- antibody. Preparations active in this assay can also initiate immune responses in B cells obtained from thymectomized, irradiated, bone marrow-reconstituted mice and in B cells separated from normal bone marrow.

Three pieces of evidence suggest that cells liberating the active factors are T cells. First, pretreatment of such a population of cells with anti- antibody abolishes its capacity to produce the activity. Second, the sedimentation profile of SRBC-stimulated factor-producing cells is identical to the sedimentation profile of T cells required to initiate anti-SRBC antibody production in vitro. Third, the sedimentation profile of factor-producing cells in a population of alloantigen-stimulated cells is identical to the sedimentation profile of cells involved in cell-mediated immunity in the same population.

Footnotes

¹ Send all correspondence to: Dr. R. A. Phillips, The Ontario Cancer Institute, 500 Sherbourne Street, Toronto 5, Ontario, Canada.

² R. M. G. was the recipient of a Commonwealth Scholarship. Present address: National Institute for Medical Research, Mill Hill, London, England.

³ Supported by the National Cancer Institute of Canada and the Medical Research Council (Grant MA-3017).