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Immunochemical Studies on Dextrans

VII. Further Characterization of the Combining Sites of Human and Rabbit Antidextrans and Horse Antipneumococcal Antibodies by Oligosaccharide Inhibition Studies with Trisaccharides of Glucose Containing (1 2) and (1 6) Linkages¹

From the Department of Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan, and the Departments of Microbiology, Human Genetics and Development, and Neurology, College of Physicians and Surgeons, Columbia University, and the Neurological Institute, Presbyterian Hospital, New York, New York 10032

Abstract

Human and rabbit antidextrans of (1 2) specificity have been further characterized as to their combining sites based on oligosaccharide inhibition assays with three trisaccharides: 1) dGlc(1 2)dGlc(1 6)dGlc, 2) dGlc·(1 6)dGlc(1 2)dGlc and 3) dGlc(1 2)[dGlc(1 6)]dGlc. After absorption of (1 6) specific antibody, the human antidextran sera contained antibodies complementary to trisaccharide 1 while the rabbit antisera contained antibodies complementary to both trisaccharides 1 and 2. With human antidextrans with non-(1 2) specificity these trisaccharides were not the best inhibitors. The various rabbit antisera studied with dextrans of predominantly (1 6) linkages showed combining site sizes with upper limits complementary to IM5, IM4 and IM3. The cross-reaction of horse antipneumococcal type XII antiserum was best inhibited by trisaccharide 1.

Footnotes

¹ This work was carried out under United States-Japan Cooperative Science Program 5R025 and with a grant from the World Health Organization (15/181/24) to M.T. and the support of the National Science Foundation (GB-25686 and GB-35243X) to E.A.K.