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A Quantitative Assay for the Mixed Leukocyte Reaction by Mouse Spleen Cells¹

From the Institute of Medical Science and Department of Medicine, University of Toronto, and the Ontario Cancer Institute, Toronto 284, Ontario

Abstract

A method for culturing mixtures of allogeneic mouse spleen cells which gives quantitative results is described. The kinetics of the response is reproducible from experiment to experiment and the dose-response curve is linear. Radiation survival studies indicate that the responding cells require the capacity to proliferate during the mixed-leukocyte reaction (D₃₇ = 95 rads). From experiments with the spleen cells of congenic resistant strains it is evident that the response is controlled by minor histocompatibility loci as well as by the H-2 locus.

Footnotes

¹ This work was supported by grants from the Medical Research Council of Canada and the Ontario Cancer Treatment and Research Foundation.

² Associate, Medical Research Council of Canada. Address all correspondence to: Dr. David Osoba, Department of Medicine, The Ontario Cancer Institute, 500 Sherbourne Street, Toronto 284, Ontario.