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A Genetically Determined Antibody Response to Poly (l-Lysine) in Guinea Pigs¹

From the Department of Chemical Immunology, the Weizmann Institute of Science, Rehovot, Israel

Abstract

The ability of poly(l-lysine) alone and poly(l-lysine) complexed to RNA to produce specific anti-poly(l-lysine) antibodies in strain 2 and strain 13 guinea pigs was tested with a sensitive complement fixation assay. The sera from all immunized strain 2 guinea pigs showed anti-poly(l-lysine) antibodies while none were detected in the sera from immunized strain 13 guinea pigs. The antibody nature of the reactive serum component was confirmed by ammonium sulfate precipitation, antiglobulin absorption and Sephadex G-200 gel filtration. The sera reacted specifically with poly(l-lysine) and not with poly (d-lysine), poly(d-arginine) or poly(l-ornithine). The results demonstrate that poly(l-lysine) itself, in adjuvant, is capable of eliciting specific anti-poly(l-lysine) antibodies and that this response is controlled by the poly(l-lysine) gene.

Footnotes

¹ This work was supported in part by Agreement 06-035 with the United States National Institutes of Health.

² Present address: The Immunology Branch of the National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014. Recipient of Fellowship IF03AM 44,020-01AL from the National Institutes of Health.

³ Recipient of Senior Fellowship of the Weizmann Institute of Science. On leave from the Department of Biochemistry and Pharmacology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111. Please send correspondence and reprint requests to this address.