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Human Leukocyte Migration Inhibition in Agarose Using Four Antigens: Correlation with Skin Reactivity^1,2,

From the Department of Pediatrics and Section of Hematology and Immunology, Department of Medicine, University of California School of Medicine, San Francisco, California 94122

Abstract

A technique is described for the in vitro evaluation of human cellular immunity by determining the inhibition of the migration of peripheral buffy coat leukocytes in agarose plates after incubation of the cells with and without antigens. The technique is simple to perform and results can be obtained in 18 hr. With three antigens (Candida, PPD and SK-SD), the results correlated well with skin reactivity in normal subjects.

Footnotes

¹ This research was aided by grants from the National Multiple Sclerosis Society (777-A-I) and from the United States Public Health Service (AI-10495, AI-00063-09, AI-09145 and HD-05894) and the Tuberculosis and Respiratory Disease Association of California (1180).

² Address reprint requests to Dr. Lynn Spitler, University of California, San Francisco Medical Center, San Francisco 94122.

Please address correspondence concerning manuscript and galley proofs to: Stephen H. Astor, M.D., Department of Pediatrics, University of California, San Francisco Medical Center, San Francisco, California 94122.

³ Recipient of a United States Public Health Service Research Career Development Award (I-K4-AI-43-012-01).