HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kennedy, J. C. Articles by Ekpaha-Mensah, J. A. Search for Related Content PubMed Articles by Kennedy, J. C. Articles by Ekpaha-Mensah, J. A.

Genetics of Stimulation of in Vitro Hemolytic Plaque-Forming Cell Responses by Irradiated Allogeneic Spleen Cells¹

From the Division of Cancer Research of the Department of Pathology, Queen's University and the Kingston General Hospital, Kingston, Ontario, Canada

Abstract

The addition of heavily irradiated spleen cells to cultures containing syngeneic spleen cells and foreign erythrocytes may stimulate to some extent the hemolytic plaque-forming cell responses of those cultures, but the addition of allogeneic irradiated spleen cells may cause much greater stimulation. Irradiated spleen cells from mice of various inbred strains were tested for their ability to produce such stimulation in cultures containing spleen cells from an allogeneic or congenic resistant partner. It was found that a genetic difference in the region of the K end of the H-2 locus was sufficient to cause strong reciprocal stimulation, while a difference in the region of the D end was not. Multiple non-H-2 histocompatibility differences were ineffective, with two interesting exceptions. It is suggested that reciprocal allogeneic stimulation of in vitro hemolytic plaque-forming cell responses may be a useful means of detecting major histocompatibility differences. A model for allogeneic stimulation is proposed.

Footnotes

¹ Research supported by grants from the National Cancer Institute of Canada.

² Research Associate of the National Cancer Institute of Canada.

³ Ghana Government Scholar.