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Isogeneic Lymphocyte Interaction: Responsiveness of Murine Thymocytes to Self Antigens¹

From the Department of Microbiology and Immunology, State University of New York, Downstate Medical Center, Brooklyn, New York 11203

Abstract

Previous studies in this laboratory have shown that thymocytes from neonatal CBA/J mice will undergo in vitro blastogenesis in culture with isogeneic spleen cells of adult origin. The present investigation demonstrates that thymocyte reactivity in this isogeneic lymphocyte interaction (ILI) is maximal at 2 to 3 days of age and declines thereafter, whereas the stimulatory capacity of spleen cells is minimal in the neonate but increases with increasing age of the cell donor. Data are also presented which suggest that soluble factors are not responsible for stimulation in the ILI. The results of breeding studies indicate that the capacity of spleen cells to stimulate in this reaction is probably controlled by more than one gene. An evaluation of the capacity of various adult lymphoid tissues to stimulate in the ILI indicates that cells from the spleen and to a lesser extent from lymph nodes are capable of stimulation while those from the thymus and bone marrow are devoid of stimulatory activity. Finally, an examination of several strains of mice indicates that most exhibit weak but positive responses in the ILI.

Footnotes

¹ This Investigation was supported by United States Public Health Service Grant AI-10158-01.

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