HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ishizaka, K. Articles by Okudaira, H. Search for Related Content PubMed Articles by Ishizaka, K. Articles by Okudaira, H.

Reaginic Antibody Formation in the Mouse

II. Enhancement and Suppression of anti-Hapten Antibody Formation by Priming with Carrier¹

From the Departments of Medicine and Microbiology, The Johns Hopkins University, School of Medicine, and the Good Samaritan Hospital, Baltimore, Maryland 21239

Abstract

DBA/1 mice were immunized with various doses of dinitrophenyl-ovalbumin (DNP-OA) precipitated with aluminum hydroxide gel, and both the reaginic (IgE) and IgG antibody responses were observed. Mice immunized with a minimal dose of DNP-OA, i.e., 0.02 to 0.05 µg, showed a persistent formation of anti-DNP IgE antibody, while an increase of immunizing dose resulted in only a transitory response. Formation of anti-OA IgE antibodies was persistent when animals were immunized with 0.02 to 1 µg of DNP-OA. Anti-DNP IgG antibody appeared in the serum within 10 days after immunization and reached a maximum by 2 weeks, whereas the anti-OA antibody titer increased slowly. The concentration of anti-DNP IgG antibody was much higher than anti-OA antibody during the first 3 weeks. The results showed that an optimal dose of immunizing antigen for maximal response differed with the immunoglobulin class of antibodies and antigenic determinant, even though the determinants are present on the same antigen molecule.

Priming of mice with suboptimal doses, i.e., 0.02 µg to 0.05 µg, of OA for antibody formation 2 weeks before immunization with DNP-OA either enhanced or "accelerated" both IgE and IgG anti-hapten and anti-carrier antibody responses. The results indicated participation of carrier-specific helper cells in the formation of anti-hapten IgE antibody. When animals were primed with 1 to 10 µg of OA, which was sufficient for induction of both IgG and IgE antibody response, and then immunized with DNP-OA, formation of anti-hapten antibodies was suppressed. The immunization of the primed animals with the hapten-carrier conjugate resulted in secondary response of both IgE and IgG anti-carrier antibodies.

Footnotes

¹ The work was supported by Research Grant AI 10060 from United States Public Health Service and a grant from the John A. Hartford Foundation. The paper is Publication 59 from O'Neill Laboratories at The Good Samaritan Hospital.