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An Antigenic Marker for Human Thymic Lymphocytes

Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014 and Naval Medical Research Institute, Bethesda, 20014

Abstract

Murine thymocytes and thymus-derived lymphocytes possess antigens not normally represented on most other tissues. Antigens of the TL (1), Ly (2), and (3) systems can be detected by isoimmunizations with mouse strains differing at the appropriate genetic locus. By immunization of rabbits with mouse lymphoid cells, followed by appropriate absorption procedures, reagents have been produced which can also identify and selectively lyse several different lymphocyte populations (4–6). The availability of immunologic reagents for detecting antigenic differences has greatly facilitated investigations of functional differences among subpopulations of mouse lymphocytes. Similar reagents have not been available previously for the study of human lymphocyte subpopulations. We have investigated the usefulness of absorbed heterologous antihuman thymocyte serum for the detection of antigenic heterogeneity in human lymphoid cells.

Thymus cells were obtained from fetuses and from infants under 1 year of age. Rabbits were injected in the foot pads with 10⁹ cells emulsified with complete Freund's adjuvant.