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The Effect of in Vivo Irradiation on Macrophage Function¹

Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

In vivo irradiation (660 R) of donor mice before removal of their peritoneal macrophages caused activation of these cells as indicated by increases in lysosomal enzymes but did not significantly affect the manner in which these cells handled keyhole limpet hemocyanin (KLH) in comparison to how macrophages from unirradiated donors handled KLH. The parameters measured included uptake, catabolism, retention and amount of membrane-bound KLH. In syngeneic recipients, however, the immunogenicity of equal amounts of KLH associated with activated macrophages from irradiated donors was significantly increased compared to that of KLH associated with macrophages from unirradiated donors. Also, addition of activated non-KLH-containing macrophages to the inoculum of normal KLH-containing macrophages increased the immune response. Infection of mice with lactic dehydrogenase virus also enhanced the immunogenicity of KLH associated with macrophages from these mice compared to control, uninfected mice.

Footnotes

¹ This is publication 620 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California. This research was supported by Atomic Energy Commission Contract AT(04-3)-410 and United States Public Health Service Grant AI-07007.

² Recipient of United States Public Health Service Training Grant 5T1GM683. Present address: Department of Surgery, University of Minnesota, Minneapolis, Minnesota 55455.