HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hurst, M. M. Articles by Bennett, J. C. Search for Related Content PubMed Articles by Hurst, M. M. Articles by Bennett, J. C.

Isolation and Characterization of the Glycopeptides from a Human Immunoglobulin M¹

Department of Microbiology and the Division of Clinical Immunology and Rheumatology of the Department of Medicine, School of Medicine, University of Alabama in Birmingham, Birmingham, Alabama 35294

Abstract

Five glycopeptides, designated CHO₁ through CHO₅, have been isolated from the heavy chain of a Waldenström IgM. Their approximate location in the molecule, their amino acid sequence and carbohydrate composition have been determined.

Cleavage of the heavy chain with cyanogen bromide (CNBr) produced eight fragments, four of which were found to contain carbohydrate. CHO₁ was found in CNBr-3, located toward the COOH-terminus of Fab (trypsin 60°). CHO₂ was isolated from CNBr-4 located at the NH₂-terminal end of Fc (trypsin 60°). CHO₃ and CHO₄ were found in CNBr-5 which is located in the Fc (trypsin 60°) portion of the molecule. CHO₅ was isolated from CNBr-7 which is near the COOH-terminus of the heavy chain.

The carbohydrate composition of CHO₁ was one fucose, three mannose, one galactose and two glucosamine residues. CHO₂ consisted of one fucose, six to seven mannose, two galactose and three glucosamine residues. CHO₃ contained two mannose, one galactose and two glucosamine residues. CHO₄ and CHO₅ were of simpler composition than the other three oligosaccharides, each containing three to four residues of mannose and one to two residues of glucosamine.

Footnotes

¹ These studies were supported by grants from the National Institutes of Health, Bethesda, Maryland and the John A. Hartford Foundation, Inc., New York.

² Current address: Czechoslovak Academy of Sciences, Institute of Microbiology, Department of Immunology, Prague, Czechoslovakia.