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Studies on Antibody Induction in Vitro

I. The Nature of the Primary Response to Soluble T2 Antigens by Rabbit Spleen Cells¹

Department of Immunology, The Public Health Research Institute of The City of New York, Inc., New York, New York 10016

Abstract

Soluble extracts of T2 phage prepared by detergent treatment (S-T2) stimulate the formation of vigorous primary type responses in suspensions of rabbit spleen cells. Neutralizing antibody activity in the tissue culture fluids is complement-dependent and reaches a peak at 5 days of culture after a 1-day lag period when an optimal immunogenic dose of antigen is used. Responses of spleen cells from individual rabbits vary considerably, but replicate cultures from a single spleen show excellent reproducibility. Antibodies produced in vitro with cells of unprimed rabbits belong predominantly to the IgM class, while the secondary in vitro response is mostly IgG. The suppressive effect of the inhibitors vinblastine and rifampicin on the primary response to S-T2 suggests that both RNA and DNA synthesis are required for antibody formation in this system.

Footnotes

¹ This work was supported in part by Grant GB-29018X from the National Science Foundation and Training Grant AJ00408, NIH, Bethesda, Md.

² Present address: Department of Biology, Long Island University, Brooklyn, New York 11201.