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Common Enterobacterial Antigen

I. Isolation and Purification from Salmonella Typhosa 0:901¹

Departments of Microbiology and Medicine, Boston University School of Medicine, Boston, Massachusetts

Abstract

Common enterobacterial antigen (CA) was isolated from extracts of Salmonella typhosa 0:901 by chromatography on Sephadex G-200 and by electrophoresis. Purified CA inhibited passive hemagglutination of erythrocytes coated with crude CA preparations and could be demonstrated in agar gel precipitin reactions. The purified CA preparations failed to sensitize erythrocytes for hemagglutination and were not immunogenic in rabbits. A pyrogenic response was observed with large quantities (250 and 500 µg) of CA. CA was detected in Ra, Rb and Rc mutants of Salmonella minnesota, but not in Rd and Re mutants. Preliminary chemical analyses indicate that carbohydrate is the major constituent of CA but the absence of heptose, phosphate and ketodeoxyoctonate indicate that CA is not related to the inner core of Salmonella lipopolysaccharide.

Footnotes

¹ This work represents a portion of a dissertation submitted by Margaret A. Johns to the Graduate School, Boston University, in partial fulfillment of the requirements for a Ph.D. degree. This study was supported by United States Public Health Service Grants AI-09584 02, AI-05901-08 and 5P01-HE07299-11 and a United States Public Health Service Training Grant 5 T01-AI-213-11.