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A Rabbit Anti-p-Azobenzenearsonate Antibody of Restricted Heterogeneity with Light Chains of b5 Allotype¹

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases; Laboratory of Cell Biology, National Cancer Institute; Laboratory of Microbiology and Immunology, National Institute of Dental Research; National Institutes of Health, Bethesda, Maryland 20014

Abstract

A rabbit, partially suppressed for the expression of b5-kappa-type light chains when first immunized, produced anti-p-azobenzenearsonate antibodies at 8, 13, 34 and 41 months of age, after each of four courses of immunization with an edestin-azobenzenearsonate conjugate. The antisera obtained after the second, third and fourth courses of immunization contained antibodies of limited structural heterogeneity. Light chains of b5 allotype were obtained from the purified antibodies. Amino acid sequence analysis of the first 21 residues at the amino terminus of the light chains indicated a single sequence for those from the third course antibody and a major sequence with limited heterogeneity from the second and fourth course antibodies.

Footnotes

¹ Presented in part at the Workshop on Myeloma Proteins, Homogeneous Antibodies and Autoimmunity, Bethesda, Maryland, January 1971. An abstract of this presentation was published (1).