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The Effect of Antibody on Antigen-Induced Lymphocyte Transformation¹

Department of Veterinary Pathology, Washington State University, Pullman, Washington 99163

Abstract

The influence of antibody on the antigen-induced in vitro uptake of tritiated thymidine by sensitive lymphocytes was determined. The cellular response of ovalbumin (OA)-sensitive lymphocytes was consistently depressed when OA was mixed with anti-OA antisera at concentrations equal to equivalence and 2 times antigen excess with one antiserum and 50 times antibody excess with another antiserum. At five other concentrations of these antisera, involving either antigen excess or antibody excess, the reaction of animals varied between depression and augmentation. Similar increases and decreases in stimulation occurred with cells sensitive to sheep RBC (SRBC) when the antigen was in the presence of anti-SRBC antiserum. Non-sensitive cells were not stimulated by immune complexes formed with OA or SRBC. Depressive complexes did not affect cell viability or response to phytohemagglutinin. Complexes composed of non-sensitizing antigen did not influence stimulation by another antigen to which the cells were sensitive. Depression also occurred when antibody was added to cultures composed of at least 97% lymphocytes. The experiments show that the in vitro response of sensitive lymphocytes may be specifically depressed or augmented by antibody.

Footnotes

¹ This work was supported in part by National Institutes of Health Grants AI-07471, AI-43138 and 5-FO-1-RR-054465.