HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Occhino, J. C. Articles by Schmid, K. Search for Related Content PubMed Articles by Occhino, J. C. Articles by Schmid, K.

Isolation of an Immunosuppressive Peptide Fraction from Human Plasma¹

Departments of Biochemistry, Surgery and Medicine, Boston University School of Medicine, Boston University Medical Center, Boston, Massachusetts 02118

Abstract

An immunosuppressive peptide fraction was isolated from Cohn fraction IV of normal human plasma by employing the low temperature-low salt-ethanol procedure of Cohn, fractional precipitation with ammonium sulfate, dialysis and ultrafiltration. The dissociation of the active peptide from an enriched protein fraction was found to be dependent upon high ionic strength and low pH. The resulting peptide fraction was electrophoretically heterogeneous although it appeared monodisperse by ultracentrifugation sedimenting with a coefficient of 0.6S. The peptide fraction was found to suppress both phytohemagglutinin-induced lymphocyte proliferation in vitro and the in vivo induction of splenic plaque-forming cells in mice immunized with sheep erythrocytes. The specific activity of this fraction was increased approximately 10-fold over that of Cohn fraction IV.

Footnotes

¹ This study was supported by National Institutes of Health Grants GM-10374, 2PO1 AM-10824, A1-08579, FR-05380, CA-12209 and AF 39,096, United States Public Health Service, Bethesda, Maryland.

² Recipient of NIDR Post-Doctoral Fellowship 5FO2 DE 44744.

³ Recipient of NIH Research Career Development Award AI-49096.