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Effect of Blocking the Active Site of an Antibody on the Expression of Its Idiotypic Determinants during Immunization¹

Department of Biological Chemistry, University of Illinois at the Medical Center, Chicago, Illinois 60680

Abstract

Antiidiotypic antibodies were elicited in rabbits by immunization with soluble complexes consisting of anti-p-azobenzoate antibody and excess (RGG)-p-azobenzoate. Nearly all of the antiidiotypic antisera, in contrast to such antisera formed in response to free antibenzoate antibody, were not inhibitable by haptens, i.e., by benzoate derivatives of low molecular weight. The results indicate that when the active site is blocked during immunization, antiidiotypic antibodies are directed exclusively to determinants outside the active site. The antigen, RGG-p-azobenzoate, evidently is not removed from the antibenzoate antibody before the recognition process in the recipient animal. The immunogenicity of soluble complexes is lower than that of the free antibody, as indicated by the relatively smaller percentages of donor antibody precipitable by antiidiotypic antisera. This also was attributed to blocking of an important idiotypic determinant.

Footnotes

¹ This work was supported by National Institutes of Health Grants AI-06281 and AI-10220.

² Recipient of an Arthritis Foundation postdoctoral fellowship.