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Production of Migration Inhibitory Factor by Non-Dividing Lymphocytes¹

Allergy-Immunology Service, Walter Reed General Hospital, Washington, D. C. 20012

Abstract

Lymphocyte proliferation in response to antigen was essentially eliminated by treatment with 5-bromo-2-deoxyuridine and light. The remaining lymphocytes were restimulated with the same antigen and the production of migration inhibitory factor (MIF) was determined. Under these conditions, MIF activity was still present. In addition, the absence of dividing cells did not appear to diminish significantly the amount of MIF produced.

Footnotes

¹ Presented in part at the Federation of American Societies for Experimental Biology, Atlantic City, New Jersey, April 9–14, 1972. This work was supported by a grant from the United States Army.