HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Harrison, M. R. Articles by Green, I. Search for Related Content PubMed Articles by Harrison, M. R. Articles by Green, I.

Polycarbonate Sieve Filtration of Lymph Node Cells: Selective Depletion of Immunoglobulin-Bearing Lymphocytes

From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

The physical properties of lymphocytes are likely to be heterogeneous as are their functional properties. Thus, thymus-derived (T)¹ lymphocytes are functionally distinct from bone marrow-derived (B) lymphocytes and this distinction is reflected not only in the presence or absence of easily detectable immunoglobulin on the cell surface (1), but also in the buoyant density of these cells as determined by velocity sedimentation (2) and their surface charge as measured by electrophoretic mobility (3, 4).

The lymphocyte's ability to migrate across post-capillary venules (5) may depend on general physical properties such as cell size and membrane charge adhesiveness and deformability. T lymphocytes differ from B lymphocytes in their migration and recirculation patterns (2, 6). T cells gain access to extravascular spaces such as chylous effusions more easily than B cells (7). The elegant studies of erythrocyte deformability as measured by passive filtration through polycarbonate sieves of defined pore size (8–11) led us to adapt this technique to the study of lymphocytes.

Footnotes

¹ Abbreviations used in this paper: T, thymus derived; B, bone marrow derived; LNC, lymph node cell.