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Amplification of Thymus-Influenced Lymphocytes by Poly(A:U): Inhibition of Antigen Binding by Antiserum to Immunoglobulin Light Chain^1,2,

From the Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Parkville, Victoria, 3050, Australia

Abstract

Immunization of CBA mice with sheep erythrocytes (SRBC) plus a non-toxic adjuvant consisting of complexes of the homoribopolymers of polyadenylic acid and polyuridylic acid (poly(A:U)) induces the formation of a population of immunocytoadherent cells which is enriched in thymus-derived lymphocytes (T-cells). Four days after immunization, approximately 50% of specific rosette-forming cells (RFC) from mice given SRBC plus poly(A:U) bear the antigenic marker. In contrast, the number of -positive RFC in animals given SRBC alone does not constitute a significant proportion of the population. The capacity of both immune lymphocyte populations to bind SRBC is abrogated by treatment with antiserum specific for the light chain of normal mouse immunoglobulins. The inhibitory activity of this antiserum is completely blocked by addition of purified mouse G immunoglobulin.

These observations are consistent with the hypothesis that immunoglobulin functions as the receptor for antigen on the surfaces of T-cells induced by antigen and poly(A:U).

Footnotes

¹ This research was supported by the Australian Research Grants Committee, The National Health and Medical Research Council of Australia, and the American Heart Association (72–1050).

² This is paper No. 1648 from the Walter and Eliza Hall Institute of Medical Research.

³ Postdoctoral Fellow of the Damon Runyon Cancer Society.

⁴ Special Fellow of the National Institute of Arthritis and Metabolic Diseases. Present address: Department of Surgery, The Good Samaritan Hospital, 5601 Loch Raven Blvd., Baltimore, Md. 21239.

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