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Chicken A: Physicochemical and Immunochemical Characteristics¹

From the Departments of Medicine and Pathology, McMaster University, Hamilton, Ontario, and Department of Medicine and Institute of Immunology, University of Toronto, Toronto, Ontario, Canada

Abstract

Column chromatography on Sephadex G-200 of ammonium sulfate preparations of chicken bile and duodenal washings revealed A in a high molecular weight form with elution immediately after the void volume. A from intestinal secretions was further purified by recycling chromatography on a Bio-Gel A-5M column. A constant S value was not obtained for the A, values of 11.9 to 16.2S being recorded under various conditions. The sedimentation coefficient was relatively unaltered by mild reductive procedures with 0.1 M -mercaptoethanol and 0.005 M dithiothreitol. Serum A was partially purified by ammonium sulfate precipitation and DEAE chromatography. The majority of serum A was in a high molecular weight form, eluting from a G-200 column mainly at the void volume. A smaller amount of A eluted in the 7S region. The addition of ¹²⁵I-labeled human secretory component (SC) to fresh chicken serum and subsequent sucrose gradient ultracentrifugation resulted in a 10 to 17% shift of radioactive counts to the heavier portions of the gradients. Radioautography of these fractions with heavy chain specific antisera revealed binding to A in 3/3, to M in 1/3 and to G in 0/3 experiments. Addition of human SC to biliary A did not result in binding to the A molecule. This suggests that chicken A obtained from secretions may possess its own form of SC.

Footnotes

¹ This work was supported by the Medical Research Council of Canada.

² MRC Scholar.