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Differential Sensitivity of Bone Marrow and Thymus Cells to Actinomycin D in the in Vivo Response of Mice to Sheep Erythrocytes¹

From the Department of Microbiology, University of Colorado Medical Center, Denver, Colorado 80220

Abstract

Three cell types required for the hemolytic antibody response have been defined based upon their differential sensitivities to treatment with actinomycin D: a) the thymus-derived cell that is insensitive to treatment with 1.0 µg/ml of actinomycin; b) the antibody-forming precursor cell, of bone marrow origin, that is inhibited by 0.1 to 1.0 µg/ml of actinomycin and c) an auxiliary cell that is extremely sensitive to treatment with 0.01 µg/ml of actinomycin. Utilizing these sensitivities to actinomycin we determined the repopulation of each of the three cell types in the irradiated mouse spleen.

Footnotes

¹ This work was supported by NIH Grant AI-03 047.

² Present address: Biology Department, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.