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Suppression of the in Vitro Anamnestic Response of Rabbit Lymph Node Cells by Anti-IgG Serum¹

From the Department of Microbiology, Schools of Medicine and Dentistry, Georgetown University, Washington, D. C. 20007

Abstract

Treatment of previously primed rabbit popliteal lymph node cell suspensions with specific, absorbed anti-IgG serum resulted in significant suppression of the in vitro anamnestic response to keyhole limpet hemocyanin (KLH). Anti-IgM treatment did not interfere with antibody formation. The degree of suppression observed was dependent on the amount of anti-IgG added to the cultures. High dilutions of anti-IgG consistently enhanced antibody synthesis. Maximum suppression was achieved when the anti-IgG was added within the first 12 hr after culture initiation. For effective suppression, the anti-IgG had to be in the cultures for up to 48 hr. Suppression could be reversed by the addition of purified rabbit IgG to the cultures, but not by an excess of KLH. Although anti-IgG was cytotoxic in the presence of complement, this does not appear to be the mechanism of action. The results suggest that cells involved in the anamnestic response to KLH bear a surface receptor which is a reflection of the immunoglobulin product ultimately synthesized.

Footnotes

¹ This work was presented in part at the 56th Annual Meeting of the American Association of Immunologists in April 1972 at Atlantic City, New Jersey and was supported by Grants from the Research Corporation and Washington Heart Association.