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Chicken Antibodies to Group a Streptococcal Carbohydrate

From the Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana, 70112 and the Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville, Florida, 32601

Abstract

Adult chickens were hyperimmunized with group A streptococcal vaccine in an attempt to elicit the formation of high levels of antibody of restricted heterogeneity. All birds produced precipitating antibody after 3 weeks of immunization. The highest concentration was 8.6 mg/ml. After 6 weeks of immunization the highest level was 13 mg/ml and even after 6 weeks most birds had both IgM and IgY. Approximately 10% of specifically purified antibody preparations contained some IgA. In each case this was associated with the macroglobulin fraction of Sephadex G-200. Of the 37 different antibody preparations examined only 5% (two IgM preparations) showed a clear restriction in L chain heterogeneity. These data indicate that like rabbits and sharks, chickens can produce high levels of antibody to a group A streptococcal carbohydrate with some antibody preparations showing restricted heterogeneity.

Footnotes

¹ Supported by a grant from the John A. Hartford Foundation, Inc. and National Science Foundation Grant GB-31001.

² Supported by National Science Foundation Grant GB-29095.