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The Effect of Heat and Hydrogen Ion Concentrations on the Extract of Short Ragweed

From the Medical Clinic of the Johns Hopkins University School of Medicine

Abstract

1. An extract of short ragweed pollen was subjected to heat—100°C. for periods of time ranging from one to twenty minutes. The extract was heated in concentrated solution and then diluted; it was also diluted and than heated. These dilutions were than titrated intradermally in patients suffering from ragweed hay-fever, and the reactions were studied by comparison. Variation of the reactions in a mild sufferer and in a severe sufferer of hay-fever was observed.

2. An extract of short ragweed pollen was subjected to varying degrees of heat: 60°C., one hour; 100°C., one hour; and autoclaved 15 pounds pressure, twenty minutes. This abtract was diluted: one part to 1000, one part to 10,000 and one part to 100,000; the intradermal reactions were studied and compared.

3. An adjustment of the neutral ragweed extracts to pH 8.4 and 4.0 was made; these solutions were diluted, heated as described above and then titrated in ten patients.