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Deae-Dextran and Polybrene Cation Enhancement and Dextran Sulfate Anion Inhibition of Immune Cytolysis¹

From the Laboratory of Tumor Virus Research, Institute of Medical Microbiology, University of Copenhagen, 22 Juliane Maries vej, DK-2100 Copenhagen Ø, Denmark

Abstract

The polycations DEAE-dextran and polybrene enhanced the sensitivity of the dye exclusion cytotoxic test performed on mouse spleen cells, whereas the anion dextran sulfate had an inhibitory effect.

Dextran sulfate prevented the enhancing effect of the polycations when admixed before incubation of the cells and reversed the polycation effect when added after the sensitization had taken place.

The in vitro infection of fibroblasts with murine leukemia virus was largely influenced in the same way, indicating a similarity of the membrane processes involved.

Footnotes

¹ This work was supported by grants from P. Carl Petersens Fond, Daell Fonden, Anders Hasselbalcks fond til leukaemiens bekaempelse, the Danish Medical Research Council, and the Danish Fund for the Advancement of Medical Science.