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The in Vivo Activity of in Vitro Immunized Mouse Thymocytes

II. Rejection of Skin Allografts and Graft-Vs-Host Activity¹

From the Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Melbourne, Australia

Abstract

"Cortisone resistant" CBA mouse thymocytes activated in vitro against allogeneic spleen cells and capable of lysing allogeneic target cells in vitro were assayed for their capacity to mount cell-mediated immune responses in vivo. These in vitro activated thymocytes (against H-2^d alloantigens) induced the rejection of BALB/c (H-2^d) skin grafts from T cell-depleted CBA mice. 10⁷ or more cytotoxic lymphocytes (CL) caused rejection within 12 days from T cell-depleted mice but failed to reject grafts on lethally irradiated mice.

As compared to normal "cortisone resistant" thymocytes, in vitro activated CL were inefficient in causing splenomegaly on injection into neonatal (CBA x BALB/c)F₁ mice. However, CL mounted twice the mitotic response of "cortisone resistant" thymocytes when injected into adult lethally irradiated (CBA x BALB/c)F₁ mice. The results indicate that there exists no direct relationship between the capacity of thymocytes to act as cytotoxic effector cells and to induce splenomegaly in neonatal F₁ hybrid mice.

Footnotes

¹ This work was supported by the National Health and Medical Research Council, Canberra, and Grant No. AM 11234-05 of the United States Public Health Service and AI-O-3958 of the National Institutes of Health. This is Publication 1731 from the Walter and Eliza Hall Institute of Medical Research.

² Postdoctorate Fellow of Medical Research Council of Canada.

³ Postdoctorate Fellow of the Deutsche Forschungsgemeinschaft (Grant WA 246/1). Requests for reprints should be addressed to Dr. Wagner, Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Melbourne 3050, Australia.