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Immunocytology of Cultured IgM-Forming Cells of Mouse

I. Requirement of Phagocytic Cell Factor for the Growth of IgM-Forming Tumor Cells in Tissue Culture

From the Department of Pathology, Institute for Virus Research, Kyoto University, Kyoto, Japan

Abstract

An attempt was made to gain an established culture cell line of murine IgM myeloma cells (MOPC-104E). The growth of the tumor cells could be maintained for more than 10 months in tissue culture by adding a factor obtained from the culture medium of tissue macrophages (histiocytes) or spleen phagocytic cells of mouse. The factor is protein in nature and has a molecular weight of about 5 x 10⁴ daltons. Without this factor the tumor cells stopped growing at G₁ stage and did not continue the cell cycle; however, the synthesis of IgM and its subunit was unaffected. The factor reinitiated DNA synthesis almost synchronously with a lag of about 90 min. The factor was also found in mouse serum and its activity increased remarkably after the injection of Freund's complete adjuvant. Adenosine 3':5'-cyclic monophosphate could substitute for the factor in a range of 0.3 µg/ml to 1.0 µg/ml. The immunologic role of the factor is discussed.

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