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The Journal of Immunology, 1972, 109: 968-973.
Copyright © 1972 by The American Association of Immunologists, Inc.
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Measurement of Antibody Release from Single Cells. II1

Raymond N. Hiramoto, N. Marlene Hamlin and Jerry R. McGhee

From the Department of Microbiology and the Institute of Dental Research, University of Alabama Medical Center, Birmingham, Alabama 35233

Abstract

A method for determining the relative amounts of antibody released from single cells in a population is presented. The procedure is based on estimating the minimal amount of antibody molecules required to give complete lysis in a given plaque volume. The results show that the average cellular rate from five populations studied was 360 molecules/cell/sec. The value is regarded as a minimum estimate of the average rate.

Footnotes

1 This work was supported by Grant GB-13804 from the National Science Foundation and the American Cancer IN-66J Grant.






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