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The Journal of Immunology, 1972, 109: 961-967.
Copyright © 1972 by The American Association of Immunologists, Inc.
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Measurement of Antibody Release from Single Cells. I1

Raymond N. Hiramoto, Jerry R. McGhee and N. Marlene Hamlin

From the Department of Microbiology and the Institute of Dental Research, University of Alabama Medical Center, Birmingham, Alabama 35233

Abstract

A method of measuring the rate of release of antibody by a single cell is extremely important for population studies at the cellular level. The hemolysis in gel procedure is used in these studies in an attempt to quantitate the average rate of IgM hemolysin released and the amount of antibody released by a single cell. Measurements of individual cell release in a population are made under conditions similar to the radial hemolysis in gel system. Average plaque-forming units (pfu) diameters of the populations increased linearly up to 30 min in the system and followed a normal distribution at a given time. The rate of pfu appearance and the rate at which pfu lose antibody-forming capability are also determined. In 10 separate experiments where pools of three to four animals per experiment were used, the rate ranged from 437 to 1027 molecules/cell/sec with an average rate of 700 molecules/cell/sec.

Footnotes

1 This work was supported by Grant GB-13804 from the National Science Foundation and the American Cancer IN-66J Grant.

In this and the following paper the term "release" is used to indicate antibody outside of, and produced by, a single cell. Our measurements cannot distinguish between synthesis (of molecules) and secretion of preformed antibody and it is not our intent to suggest this in these papers.






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