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The Effect of Enzyme Digestion on the Antigenicity and Biologic Activity of Clq

From the Institute of Immunology and Rheumatology, Rikshospitalet, Oslo 1, Norway

Abstract

The human complement component Clq was found to be sensitive to the action of pepsin and papain. Treatment with these two enzymes under certain conditions caused a marked decrease in the ability of Clq to agglutinate latex particles coated with IgG. Gel diffusion studies also showed the presence of components antigenically deficient when compared to non-treated Clq. After ultracentrifugation of the pepsin-treated Clq the antigenically deficient components were located in fractions having much lower sedimentation rates than native Clq.

Reduction and alkylation of Clq also resulted in decreased ability to agglutinate the latex particles and caused the formation of antigenically deficient components. However, the components with lower sedimentation constants than Clq were detected only if conditions causing decreased non-covalent interaction were utilized.