HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Miller, C. Articles by DeWitt, C. Search for Related Content PubMed Articles by Miller, C. Articles by DeWitt, C.

Rat Alloantibody Responses against Strong and Weak Histocompatibility Antigens¹

From the Department of Pathology, University of Utah Medical Center, Salt Lake City, Utah 84112

Abstract

Rat alloantisera were prepared in inbred strains of rats by immunization with lymphoid cells of a donor strain. Six host-donor combinations were incompatible at the major (AgB or H-1) as well as other histocompatibility loci. These sera contained complement dependent cytotoxins after two injections of 1 to 3 x 10⁷ cells. Titrations showed 100% kill at several antibody (ab) concentrations and a typical doubling dilution end point. Six host-donor combinations were incompatible only at non-AgB (non-H-1) loci. These sera contained cytotoxins only after two injections of 4 x 10⁸ cells. Titrations showed end points equal to those of the first group of antisera but no concentration was able to kill 100% of available target cells, nor did increments in antiserum concentration yield any change in the low number of cells killed. These characteristics were not the result of: a) the injection schedule; b) presence of complement inhibitors; c) low response capacity of the host; d) the presence of two lymphocyte subpopulations in the target cell suspension nor e) low ab concentrations. Absorption of the two types of antisera by homologous cells suggests that the non-AgB antisera: a) contain both complement-fixing (CF) and non-CF ab; b) the non-CF concentration is as much as 10-fold higher than CF; c) avidities of non-CF and CF are similar. We infer that immunization with non-AgB antigens preferentially induces formation of non-CF ab which is responsible for the odd titration and is enhancng ab.

Footnotes

¹ This work was supported by United States Public Health Research Grant AI-08934.

² Presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy, University of Utah.

³ Address correspondence to Dr. C. W. DeWitt, College of Medicine, Department of Pathology, University of Utah Medical Center, Salt Lake City, Utah 84112.