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Detection of Additional Antigenic Determinants of Hepatitis B Antigen¹

From the Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D. C. 20012

Abstract

The heterogeneous antigenic determinants of Hepatitis B antigen (HB Ag) from 310 individuals were compared by using a micro-Ouchterlony immunodiffusion technique. Reference antisera were made in rabbits immunized with a purified HB Ag with ay determinants from an American with hepatitis and another purified HB Ag with ad determinants from a Thai blood donor. The immunizing antigens were prepared by a combination of equilibrium centrifugation in cesium chloride and rate zonal centrifugation in sucrose gradients. Three immunodiffusion patterns of antigen reactivity were observed: pattern 1 antigens showed identity to the reference ay HB Ag; pattern 2 antigens showed identity to the reference and HB Ag and pattern 3 antigens showed partial identity to both reference antigens. Further study after absorbing the antisera with heterologous antigens indicated that the reference antisera were distinguishing two additional antigenic determinants, designated w and r. Preliminary data suggest w is more common that r on HB Ag in the United States, but r is most common in Thailand.

Footnotes

¹ In conducting the research described in this report, the investigators adhered to the "Guide for Laboratory Animals Facilities and Care," as promulgated by the Committee on the Guide for Laboratory Animal Facilities and Care of the Institute of Laboratory Animal Resources. National Academy of Sciences, National Research Council.

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