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The Journal of Immunology, 1972, 109: 807-809.
Copyright © 1972 by The American Association of Immunologists, Inc.
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C3 Shunt Activation in Human Serum Chelated with EGTA1

Douglas P. Fine, Samuel R. Marney, Jr., Daniel G. Colley, John S. Sergent and Roger M. Des Prez2

From the Departments of Medicine and Microbiology, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee 37232

Abstract

Complement (C) fixation via the C3 shunt, as exemplified by zymosan (Z) and Escherichia coli (E. coli), requires ionized magnesium but not ionized calcium and proceeds in the presence of 10 mM ethyleneglycoltetraacetic acid (EGTA). C-fixation via the C142 pathway requires calcium and is inhibited by EGTA. Both pathways of C-activation are inhibited by ethylenediaminetetraacetic acid (EDTA). EGTA serum may be a useful and simple reagent to distinguish between C142 and C3 shunt-mediated complement activation.

Footnotes

1 This work was supported by VA Infectious Diseases Training Program TR 74, VA Research Part I Funds, and Grant HE 08399 from the National Institutes of Health, United States Public Health Service.

2 To whom requests for reprints should be addressed.




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