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From the Department of Microbiology and Immunology, Temple University Health Sciences Center, Philadelphia, Pennsylvania
Abstract
The effects of different carriers on the anamnestic response to the DNP-ligand were investigated at the level of circulating antibody and of the single antibody-forming cell (PFC) by a modified hemolysin in gel technique with a number of different indicator sheep red blood cells (SRBC).
In all groups of mice it was found that regardless of the primary antigen the highest number of direct and indirect PFC was obtained on rechallenge with 2,4-dinitrophenyl hemocyanin (DNP-Hcy). The greatest difference between the different groups appared in the number of indirect PFC. The number of PFC after second challenge seemed dependent on the immunogenicity rather than the homology of the carrier used for challenge. Spleen weights were proportional to cell counts but generally did not correlate with the PFC response.
The indicator cell played a crucial role in the number of PFC detected: SRBC coupled with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl DNP-bovine serum albumin (ECDI-DNP-BSA) detected the largest number of cells, presumably those secreting both high and low affinity antibody, while both EDCI-DNP-mouse serum albumin and 1-fluoro-2,4-dinitrobenzene (DNP-F) coupled with SRBC detected fewer cells, presumably only those of high affinity.
It was found that circulating antibody titers were higher in DNP-human 
globulin (DNP-HGG) and DNP-Hcy-primed mice when challenged with the homologous rather than the heterologous conjugate. However, no significant differences were observed in the DNP-BSA-primed group between homologous and heterologous challenge. Antibody concentrations were highest in DNP-HGG-primed and challenged mice but differed little in the other groups. There was little correlation between antibody concentration measured by radioimmunoassay, hemagglutination (HA) titers and the number of direct or indirect PFC.
Affinities varied insignificantly among the different groups but the heterogeneity index varied significantly (0.05 
; p ; 0.005). The heterogeneity indices were highest after heterologous and lowest after homologous challenge. These differences were statistically significant and are indicative of a more homogeneous antibody population after heterologous challenge.
Footnotes
1 Supported by American Cancer Society Grant T-488A and Career Development Award Grant 1-K3-CA-19,409 to H.F.H.
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