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Purification of Human Lymphotoxin¹

From the Departments of Pathology, School of Veterinary Medicine, Davis, California 95616 and the School of Medicine and The Cancer Research Institute, University of California, San Francisco, California 94122

Abstract

This paper describes the first preparation of highly purified lymphotoxin, a soluble cytotoxin produced by lymphocytes. Lymphotoxin was produced in vitro by phytohemagglutinin-stimulation of human lymphocytes and was then purified greater than 2000-fold by a sequence of column chromatography (Sephadex G-150 followed by DEAE-cellulose) and preparatory acrylamide gel electrophoresis. Ten per cent of the cytotoxic activity was recovered in the final product. The results of the purification steps were evaluated by a new quantitative assay for cytotoxic activity.

Footnotes

¹ This work was supported by United States Public Health Service Grants CA-07191-08 and GM-15934-04.

² Supported by Training Grant TO1 GM-00537 from the National Institutes of Health, and United States Public Health Service Special Fellowship 1 FO3 AM-49756-01. Current address: Department of Experimental Pathology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California 92037.

³ Address reprint requests to Dr. Werner Rosenau, Department of Pathology, University of California School of Medicine, San Francisco, California 94122.