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Immunoglobulins on the Surface of Lymphocytes

V. Quantitative Studies on the Question of Whether Immunoglobulins Are Associated with T Cells in the Mouse¹

From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206

Abstract

The presence of immunoglobulin (Ig) on the surface of lymphocytes that were negative for Ig by immunofluorescence was investigated by combining a quantitative inhibition assay with immunofluorescence. Thymus cells; thymus, spleen and lymph node cells that had been passed through anti-Ig-coated plastic bead columns; and -positive mouse lymphomas were evaluated by using these two systems. These cell preparations had 0 to 1% of cells with detectable surface immunoglobulin by immunofluorescence. If the assumption is made that the fluorescent-positive cells were relatively homogeneous with respect to the amount of surface Ig, then it could be calculated that there was more Ig present in the various cell populations studied than was accountable by the small percentage of fluorescent-positive cells. Based on these calculations, the amount of Ig present in the different populations was found to be: fluorescent-positive cells: 5 to 14 ng N/10⁶ cells; fluorescent-negative thymus cells: 0.01 ng N/10⁶ cells; fluorescent-negative spleen and lymph node cells 0.07 to 0.1 ng N/10⁶ cells. Three of four -positive lymphomas also had detectable Ig in amounts 3 to 10 times that found in normal thymus. Routinely there was more IgM found in both fluorescent-positive and -negative normal cells than IgG2.

Footnotes

¹ This work was supported by United States Public Health Service Grant AI-09758 and by the American Heart Association Grant 70-749.

² Supported by the United States Public Health Service Training Grant AI-00409.

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