HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Trump, G. N. Search for Related Content PubMed Articles by Trump, G. N.

Preparation of Dinitrofluorobenzene-Treated Sheep Erythrocytes for Detection of Anti-DNP Plaque-Forming Cells¹

From the Department of Microbiology and Immunology, University of Southern California School of Dentistry, Los Angeles, California 90007

Abstract

A method is described for covalent attachment of dinitrofluorobenzene (DNFB) to sheep red blood cells (RBC). The solubility and ionization characteristics of DNFB are considered in this procedure and relatively large amounts of hapten are reacted with RBC. Unreacted DNFB is partially precipitated and soluble DNFB is carefully removed from DNP-RBC at the end of reaction. Haptenated erythrocytes are extremely sensitive to hemolytic antibody in the direct agarplaque assay as well as in passive hemolysis. Previously reported DNFB techniques were carried out in parallel experiments and results of such comparisons highly recommend the present method for use in detection of direct anti-DNP plaque-forming cells.

Footnotes

¹ This work was supported by United States National Institutes of Health Grant AI-10112-01 ALY.