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A Mechanism for the Unresponsive State in BALB/c Mice after Repeated Doses of Sheep Erythrocytes¹

From the Immunobiology Unit, Department of Botany and Microbiology, Montana State University, Bozeman, Montana 59715

Abstract

BALB/c mice were rendered unresponsive to sheep red blood cells (SRBC) by injections of 5 x 10⁸ SRBC every other day from 7 days of age to adulthood. It was shown that unresponsiveness was specific by injection of burro red blood cells which produced normal plaque-forming cell responses in SRBC-unresponsive mice. Neither normal thymus nor bone marrow alone or in combination, nor normal peritoneal exudate cells (80 to 90% macrophages) reconstituted unresponsive mice. However, SRBC-primed peritoneal exudate cells (80 to 90% macrophages) did reconstitute the plaque-forming response of unresponsive mice. Normal BALB/c mice were rendered unresponsive by injection of 32 hemagglutinin units of serum from unresponsive mice. Serum from unresponsive mice was absorbed with IgG2. Serum absorbed in this manner failed to render mice unresponsive when administered passively. It was concluded that unresponsiveness to SRBC involves IgG2 which interacts with the macrophage and, in turn, inhibits antigen-macrophage interaction. Thus this system represents a feedback mechanism which prevents the presentation of antigen to the lymphocyte.

Footnotes

¹ This work was supported in part by United States Public Health Service Training Grant 5-T01-AI00131-09 and United States Public Health Service Grant AI06652-07.