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From the Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Abstract
A study was made on the effects of monospecific rabbit antibodies to guinea pig C2, C3 and C5 on the production and action of guinea pig lymphotoxin. Lymphotoxin was produced by incubating ovalbumin or purified protein derivative (PPD) with lymph node cells from guinea pigs which had been immunized with ovalbumin in complete Freund's adjuvant. Culture fluids from cultures of lymph node cells which had been preincubated with anti-C2, anti-C3 or anti-C5 and then incubated with both antigen and the anti-complement component antiserum were as cytotoxic when assayed on L cell monolayers as were culture fluids from cultures of lymph node cells incubated with antigen alone. Furthermore, anti-complement component antibody activity could be demonstrated in culture fluids containing lymphotoxin produced in the presence of anti-C2, anti-C3 or anti-C5 indicating that the concentrations of antisera used were sufficient to neutralize respectively all the C2, C3 or C5 present in the culture system. It is therefore concluded that the complement system, activated either via the classical pathway or via the "alternate" pathway, plays no role in the lymphotoxin phenomenon.
Footnotes
1 This work was supported in part by United States Public Health Service Training Grant M 5 TO1 AI00282-08, United States Public Health Service Research Grant 5 RO1 AI-02566-13, and National Science Foundation Grant GB7406X1.
2 Recipient of Pre-Doctoral Fellowship on United States Public Health Service Training Grant M 5 TO1 AI00282-08.
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