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Valence and Temporal Change in Affinity of Purified 7S and 18S Nurse Shark Anti-2,4 Dinitrophenyl Antibodies¹

From the Department of Microbiology, University of Illinois, Urbana, Illinois 61801, the Department of Microbiology, University of Miami, School of Medicine, Miami, Florida 33155, and Lerner Marine Laboratory, Bimini, Bahamas

Abstract

Purified shark 7S and 18S anti-2,4 dinitrophenyl (DNP) antibodies were purified by immunoadsorption. Comparative equilibrium dialysis studies with the ³H--DNP-l-lysine ligand over a 20-month period indicated that the 7S antibody showed a 100-fold increase in the average intrinsic association constant. The binding affinity of the 18S molecule did not change significantly during the same period. Valences of 1.4 and 8 were measured for the 7S and 18S molecules respectively, purified from the 30-month bleedings.

Footnotes

¹ This investigation was supported by United States Public Health Service Research Grants AI-05758 and AI-02288 from the National Institute of Allergy and Infectious Diseases. Part of the equipment used was purchased with funds provided by the American Cancer Society, Florida Division, Inc.

² Department of Microbiology, University of Illinois, Urbana, Illinois 61801.

³ Department of Microbiology, University of Miami, School of Medicine, Miami, Florida 33155, and Lerner Marine Laboratory, Bimini, Bahamas.