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Hypersensitivity to Ascaris Antigens

VII. Isolation and Partial Characterization of an Allergen¹

From the Department of Bacteriology and Immunology, University of Western Ontario, London, Canada and the Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California³

Abstract

An allergen from Ascaris suum crude extract was isolated by a combination of Sephadex G-200 gel filtration and DEAE-cellulose chromatography. The allergen was detected over a wide range of molecular weights but had the property to stimulate homocytotropic (Hc) antibody production only when present in a molecular weight range of 30,000 to 40,000. The main bulk of the immunogenic fraction was negatively charged at pH 8.6, but the entire fraction displayed an extremely broad range of electrophoretic mobilities as detected by bidimensional immunoelectrophoresis; the allergen contained 11% reducing sugars. Bordetella pertussis-treated rats produced relatively high titers of Hc antibodies when injected with as little as 10 µg of the partially purified allergen.

The allergen (Asc-1) was obtained in a highly purified form by associating Sephadex gel filtration with polyvinyl-chloride preparative electrophoresis. A monospecific rabbit precipitating antiserum against Asc-1 was capable of completely removing the allergenic activity by specific absorption.

Footnotes

¹ This investigation was supported by Medical Research Council of Canada Grant MA-3072 and by Public Health Service Research Grant AI-1339 from the National Institute of Allergy and Infectious Diseases.

² Miss Hussain is a recipient of a Canadian Commonwealth Predoctoral Fellowship.

³ Contribution 4388.