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Characterization of Migration Inhibitory Factor (MIF) from Guinea Pig Lymphocytes Stimulated with Concanavalin A¹

From the Departments of Medicine and Biological Chemistry, Harvard Medical School and the Robert B. Brigham Hospital, Boston, Massachusetts 02120

Abstract

A migration inhibitory factor (MIF) obtained from cultures of guinea pig lymphocytes stimulated with the mitogen concanavalin A (MIF_{Con A}) was characterized and compared to MIF produced by stimulation with the o-chlorobenzoylbovine globulin antigen (MIF_OCB-BGG). MIF_{Con A} behaves like MIF_OCB-BGG on acrylamide gel electrophoresis, in its buoyant density on isopycnic centrifugation, and in its susceptibility to neuraminidase and chymotrypsin. On the other hand, MIF_{Con A} showed greater heterogeneity than MIF_OCB-BGG when chromatographed on Sephadex G-100, and Con A stimulated the production of more MIF than antigen. The increase in heterogeneity and activity of MIF_{Con A} over MIF_OCB-BGG would be expected if Con A affects more cells than antigen. These experiments suggest that the triggering of different kinds of receptors results in the production of similar mediators, probably through a common pathway.

Footnotes

¹ This work was supported in part by United States Public Health Service Grants AI-07685 and GRS-RR05669 and a Grant from the John A. Hartford Foundation.

² Recipient of a Cancer Research Scholar Award from the American Cancer Society.