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The Clonal Nature of Antibody Formation

III. Independence of Antibody Cell Clones to Poly-O-Acetyl-D-Serine and Poly-D-Alanine¹

From The Institute for Cancer Research, Fox Chase, Philadelphia, Pennsylvania 19111

Abstract

Experiments were designed to test the hypothesis that prospective antibody cell clones can synthesize antibody molecules of only one specificity. This was done with the aid of a cell transfer system as follows: hapten-specific precursor cells of F₁-hybrid mice were stimulated with protein carriers bearing random copolymers O-acetyl-D-serine and D-alanine (ser-ala); antibody allotypes served as genetic markers in evaluating the clonal origins of antibodies which were specific for the homopolymer haptens, poly-ser or poly-ala. Our analysis of the results supported the conclusion that different and unrelated antibody precursor cells responded to poly-ser and poly-ala determinants. Apparently the antibody specificity of these precursor cells was genetically restricted before contact with antigen.

Footnotes

¹ This work was supported by United States Public Health Service Grants CA-04946, CA-06927 and RR-05539 from the National Institutes of Health and by an appropriation from the Commonwealth of Pennsylvania.